| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238774 |
| Chromosome: | chromosome 1 |
| Location: | 5051500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034950 | (1 of 1) K06184 - ATP-binding cassette, subfamily F, member 1 (ABCF1); Soluble ABC-F domain containing protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCTCCACAGGGCACAGGCTGCATGGCGG |
| Internal bar code: | TGAGATGATTCAGCCGGACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 98.6025 |
| LEAP-Seq n confirming: | 635 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAAGAAAAGTGCGTTC |
| Suggested primer 2: | TGGATGGTGGGGTATTAGGA |