Insertion junction: LMJ.RY0402.238777_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.238777
Chromosome:chromosome 13
Location:814184
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g567150 (1 of 1) PTHR16220:SF0 - WD REPEAT-CONTAINING PROTEIN WRAP73 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGAGGGCAGAGACAGGCGCCCCGGGCC
Internal bar code:AACCTATACTTCAGTTGAGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:107
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACAATCCCAAACAACCC
Suggested primer 2:AATCCCACCCAACTACTCCC