| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238803 |
| Chromosome: | chromosome 7 |
| Location: | 5179914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348450 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACATTCCGGTCGCGGCTAGGGTCTCGG |
| Internal bar code: | GTCATAACTTGGACTCCTTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 99.7557 |
| LEAP-Seq n confirming: | 1225 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAACGAGTAGTCCCGCTT |
| Suggested primer 2: | CTCGTGACTCGGCATTCATA |