| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238826 |
| Chromosome: | chromosome 10 |
| Location: | 6236170 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464650 | CGL36 | (1 of 1) PF12646 - Domain of unknown function (DUF3783) (DUF3783); Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATCGACATGGAGGCGGACATGGTCAAG |
| Internal bar code: | GTACTAAGCTGCTTTTCGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.3939 |
| LEAP-Seq n confirming: | 984 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAACAAGCTAGACAAGGC |
| Suggested primer 2: | CGCATTCTCCTACTTGCACA |