Insertion junction: LMJ.RY0402.238834_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.238834
Chromosome:chromosome 5
Location:186191
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g234300 (1 of 1) K12175 - COP9 signalosome complex subunit 1 (GPS1, COPS1, CSN1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCATCCGCCGCCCACCTCCGCCACGTGCA
Internal bar code:CCGCAATTTAGTGTTGTTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:94.375
LEAP-Seq n confirming:151
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGTTATATTGGCTGTGCT
Suggested primer 2:AAACGTCAACGTGACCATCA