Insertion junction: LMJ.RY0402.238844_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238844
Chromosome:chromosome 7
Location:1719180
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCAGGGCCTCAAACCATCTTGTGCTGCCC
Internal bar code:TTGGTGCGGTGTTCTCTGCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:98.1087
LEAP-Seq n confirming:415
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAAATGCTGCTCCTCACC
Suggested primer 2:AACCTTTGGAATGAAACCCC