Insertion junction: LMJ.RY0402.238876_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.238876
Chromosome:chromosome 17
Location:6800427
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g744897 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACCCCCGCCCGCGCCCCATCAAACCC
Internal bar code:CGGCATCTTAAGCTAGGCATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:20
LEAP-Seq percent confirming:95.3527
LEAP-Seq n confirming:1149
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAACGTGGACACACAGGT
Suggested primer 2:CGGGAGATCTGTGTGTGATG