| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.238899 |
| Chromosome: | chromosome 17 |
| Location: | 3183678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721900 | COG5 | Component of oligomeric golgi complex; (1 of 1) PF10392 - Golgi transport complex subunit 5 (COG5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGGCAGTGGAAGATGCGGGTGCAATCT |
| Internal bar code: | AACGTGTAGGCTTGACTAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCTTTGACTGCTTGAAA |
| Suggested primer 2: | GCCTCAGTGCGTCTCATGTA |