| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.238956 |
| Chromosome: | chromosome 6 |
| Location: | 5500373 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284800 | (1 of 1) K05541 - tRNA-dihydrouridine synthase C [EC:1.-.-.-] (dusC) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGAGACATATCAACACCAGGGAGACGA |
| Internal bar code: | CCGAGTCGCCGGCTCGGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.1031 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | AATGATGGAGCTGGAGGATG |