| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.238972 |
| Chromosome: | chromosome 9 |
| Location: | 3450845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389319 | (1 of 2) PTHR11266:SF17 - PROTEIN MPV17 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATCTTACAGCTTATATAACATTAACCA |
| Internal bar code: | GGACCTGCAGGAGTGGTAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 91.8033 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGTTGCCGAAGAACAC |
| Suggested primer 2: | GGGTGAAGTAGCGAGACGAG |