Insertion junction: LMJ.RY0402.238993_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.238993
Chromosome:chromosome 1
Location:360588
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g002201 (1 of 1) PTHR15002//PTHR15002:SF0 - UNCHARACTERIZED // RIBOSOMAL BIOGENESIS PROTEIN LAS1L 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTGTCGGTAACCTCCACTGGTCGCCAA
Internal bar code:TTTGCGAGTCTCCAACGGGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:537
LEAP-Seq percent confirming:99.3481
LEAP-Seq n confirming:762
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGAGGGTGAGAAGGAG
Suggested primer 2:CTAGGGTCGTCTTCTCGTCG