Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.239016 |
Chromosome: | scaffold 24 |
Location: | 22480 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre24.g755247 | (1 of 1) IPR003010//IPR020683 - Carbon-nitrogen hydrolase // Ankyrin repeat-containing domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCCCTAGCTCATTCTTTAGATTCTCCT |
Internal bar code: | GCGCACCGAGGGTGGGGATAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 131 |
LEAP-Seq percent confirming: | 98.5075 |
LEAP-Seq n confirming: | 66 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAAGCATGTAAGGCCGTCG |
Suggested primer 2: | GAAAACGCTTCCGCTAAGTG |