| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239025 |
| Chromosome: | chromosome 3 |
| Location: | 2720568 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161550 | FAP55 | (1 of 1) IPR009053//IPR023220 - Prefoldin // Type IV secretion system, VirB5-domain; Flagellar Associated Protein 55 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTTGCGAGGTGAGGGATTGGAAGGAGG |
| Internal bar code: | CCGTAACGTCAACTGTGGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 83.0769 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATTGCAGCTTCTGCGTC |
| Suggested primer 2: | TCAGCACGCGCTAATAAATG |