Insertion junction: LMJ.RY0402.239039_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.239039
Chromosome:chromosome 17
Location:6020940
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g740800 (1 of 5) PF06742//PF06863 - Protein of unknown function (DUF1214) (DUF1214) // Protein of unknown function (DUF1254) (DUF1254) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGGTTGGACACATTCCGCTGGCGAGTG
Internal bar code:TCTATGAGAAGAACAACAGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:480
LEAP-Seq percent confirming:99.0476
LEAP-Seq n confirming:208
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCATGCTGTTGAGCTGT
Suggested primer 2:ATAGAGGACGTGGAACACGG