| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239047 |
| Chromosome: | chromosome 7 |
| Location: | 312894 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314351 | (1 of 2) K14819 - dual specificity phosphatase 12 [EC:3.1.3.16 3.1.3.48] (DUSP12, YVH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTCGCCCCACCAACCGCATCTGCTTCT |
| Internal bar code: | CACTGATGTGGCACACACCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACACGGAGGTGTTTGGC |
| Suggested primer 2: | AGGTGATAAGCGGTCACGTC |