| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239049 |
| Chromosome: | chromosome_7 |
| Location: | 433214 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g315300 | CSG4 | Predicted protein of CSG family | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACAGGCGCGGTCGATGTCAGGCAGGCGG |
| Internal bar code: | GCGGGTCTTTGATAGCCTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 99.7758 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAAAACGCATTCTACC |
| Suggested primer 2: | GCAGCTCATTGCATAACGAA |