Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.239049 |
Chromosome: | chromosome_13 |
Location: | 3741472 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGTCGCCAACCTCAATCGTCATGGTTGAT |
Internal bar code: | GCAGGTGACTGACCGGCAATCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 522 |
LEAP-Seq percent confirming: | 98.1982 |
LEAP-Seq n confirming: | 218 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTAGCTGAACTCCACCG |
Suggested primer 2: | AACATGCCTGGTTGTAAGCC |