| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239118 |
| Chromosome: | chromosome 14 |
| Location: | 3226257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629241 | CYG25 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGCAGCCTTGCCGCTGCGACGGCGAG |
| Internal bar code: | TGTATCTGGTCGTAGAAATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 98.8304 |
| LEAP-Seq n confirming: | 338 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCACCTCTCGAACTCCG |
| Suggested primer 2: | GTGGGCCTCTTGTCAACTGT |