| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239181 |
| Chromosome: | chromosome 2 |
| Location: | 5066314 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104725 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGTGGCTTACAGCCCATCCACGCCAGC |
| Internal bar code: | CCCAGACCCGGGCGTGGACTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.0843 |
| LEAP-Seq n confirming: | 541 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCGTGAGGAGCTTGATT |
| Suggested primer 2: | CGGCTAGCATTCGCTAAAAC |