| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239186 |
| Chromosome: | chromosome_1 |
| Location: | 5467561 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g038500 | CYP746A1,CYP3 | Cytochrome P450, CYP197 superfamily, CYP197B family | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGAAGGCGCCCCCGCTGCTACTGCCGGC |
| Internal bar code: | GCGGGCGACCAAGTGGGCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.8152 |
| LEAP-Seq n confirming: | 540 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAGAGAGGTCGTGGGCTG |
| Suggested primer 2: | CTGTGCAGCCAAGTTAACGA |