| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239204 |
| Chromosome: | chromosome_12 |
| Location: | 2573396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g505350 | ACA2 | P-type ATPase/cation transporter, plasma membrane | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGAAAGGCGATGTGGAGGCTCCGGAGCC |
| Internal bar code: | GACTTAGGGGAAGAGATATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 95.4315 |
| LEAP-Seq n confirming: | 188 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTGGTTGTTTCCTGTT |
| Suggested primer 2: | TACGGACACCACAAGGATGA |