| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239222 |
| Chromosome: | chromosome 1 |
| Location: | 5454706 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038376 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCACCAGGAAGGTATCGCTGGTGCAAG |
| Internal bar code: | ACGGTCATGTTTCAAGATGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 98.8281 |
| LEAP-Seq n confirming: | 253 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGGTCCATCACCATGTTT |
| Suggested primer 2: | TATGCTGCTCTTTGGTCGTG |