| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239224 |
| Chromosome: | chromosome 17 |
| Location: | 2428667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g715600 | (1 of 1) PF11822 - Domain of unknown function (DUF3342) (DUF3342) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCCGTGGCTTCTTCTCGGGCTGGCTT |
| Internal bar code: | CTCGCGACTGGGCACCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 120 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGACATACGCCCACTACA |
| Suggested primer 2: | CTGCTGGCTGGTAGAGGTTC |