| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239228 |
| Chromosome: | chromosome 10 |
| Location: | 2635285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGACCTGCTACGCGGAATGCTGCTTGCC |
| Internal bar code: | CCCTCACGTGTAACTGAGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 112 |
| LEAP-Seq percent confirming: | 99.2754 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTAGTTGCCGGACTC |
| Suggested primer 2: | TGGCTGAAAGTTTGCTGATG |