Insertion junction: LMJ.RY0402.239244_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.239244
Chromosome:chromosome 3
Location:8045952
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCTGGCGGCGGCGGAGCCCACACCGGAG
Internal bar code:GTCCCCTGACAGAGGCATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:62
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCGGTGATTCAGTGTTT
Suggested primer 2:CCCTCAATGCTCCATGAGTT