Insertion junction: LMJ.RY0402.239244_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.239244
Chromosome:chromosome 6
Location:2166316
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g265950 DHC3 Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCGGCAGCTGCCAGCAGCTGCTCCG
Internal bar code:GTGCTTTCGACGATAGGGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:435
LEAP-Seq percent confirming:88.0342
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTCCACCAGGAACATCT
Suggested primer 2:GAGCTGTTCACCGACTCCTC