| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239276 |
| Chromosome: | chromosome 7 |
| Location: | 4704242 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344950 | LHCA9 | Light-harvesting protein of photosystem I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGAGGCTGGTCAGATCACCTGGACTCG |
| Internal bar code: | GTTGGTAAGAAGTATTGGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 99.2945 |
| LEAP-Seq n confirming: | 563 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATCCCCTGTTACATCCCC |
| Suggested primer 2: | ATTGAACCATGTGTGGAGCA |