| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239277 |
| Chromosome: | chromosome 6 |
| Location: | 1780689 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262350 | (1 of 1) IPR003722 - Cobalamin (vitamin B12) biosynthesis CobH/CbiC, precorrin-8X methylmutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACACGACAATATTGAAATGATGCGGT |
| Internal bar code: | ACGCATGGTGTAGACGGCGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 99.6245 |
| LEAP-Seq n confirming: | 796 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGCGTGT |
| Suggested primer 2: | CTTGTCGGTCGAGAGAGTCC |