| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239281 |
| Chromosome: | chromosome 5 |
| Location: | 3037961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238650 | PHC5 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGGCGGTGGCCGCGGCCCTGGAAGTGG |
| Internal bar code: | AAGTTCAGGATTTAATAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 98.6607 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGTAGGCATCACCGAA |
| Suggested primer 2: | GCCAGGAGAGCTTAATGTGC |