| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239307 |
| Chromosome: | chromosome 3 |
| Location: | 2328491 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158400 | FAP218 | Flagellar Associated Protein 218; (1 of 1) IPR023160 - Ribonuclease HII, helix-loop-helix cap domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGCGATGGGACAGGATAAGGGGCAGG |
| Internal bar code: | GGGGGGTACAATTTGGCAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 98.3425 |
| LEAP-Seq n confirming: | 178 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCATGTGCCTGGATATT |
| Suggested primer 2: | TCAAGGTTGCGTATGAGCTG |