| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.239336 |
| Chromosome: | chromosome 11 |
| Location: | 807649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467641 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGCCGCCACCGCCGCCCGCACCATCG |
| Internal bar code: | TTTTTGTTGTGCTATTTTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.3389 |
| LEAP-Seq n confirming: | 3456 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCACAGGTGGCTTTCAT |
| Suggested primer 2: | GTACTCTCCGTCTGGCAAGC |