Insertion junction: LMJ.RY0402.239336_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.239336
Chromosome:chromosome 11
Location:807649
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467641 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGCCGCCACCGCCGCCCGCACCATCG
Internal bar code:CATGTAAAAAGTGTGGGATAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:278
LEAP-Seq percent confirming:96.1073
LEAP-Seq n confirming:1111
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGATCACAGGTGGCTTTCAT
Suggested primer 2:GTACTCTCCGTCTGGCAAGC