| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239336 |
| Chromosome: | chromosome 12 |
| Location: | 5275529 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528614 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAGAGCTTGGCAAGGTCTGCGTCCCAT |
| Internal bar code: | GACGGCGACTTGACGTGCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 98.5153 |
| LEAP-Seq n confirming: | 1128 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGGAGCCAACTCAAAC |
| Suggested primer 2: | TGACGAACTCCTCCACTTCC |