| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239348 |
| Chromosome: | chromosome_5 |
| Location: | 1760235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g242500 | RSP4 | Radial spoke protein 4 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCAGGAAACTAGCCGGAGTGTTTCGTTA |
| Internal bar code: | CCAGACCGGGTACGGTATACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 99.4455 |
| LEAP-Seq n confirming: | 538 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCTCCAACACTTCTCA |
| Suggested primer 2: | ATAGTCGTGGATGAGGGACG |