| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239361 |
| Chromosome: | chromosome 4 |
| Location: | 2853929 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224350 | MOT55 | Predicted protein; (1 of 22) PTHR10994 - RETICULON | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTCTGGTCCAGGCTGGGCCGGTTGCT |
| Internal bar code: | TGAAGGAGGTTCAAACAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.6161 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGATGCGAGTAAAGAGAG |
| Suggested primer 2: | GGCCCTTATGCATCAGTTGT |