| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239363 |
| Chromosome: | chromosome 11 |
| Location: | 2749493 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477150 | (1 of 4) IPR000533 - Tropomyosin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATCGCGATTCCGCCGCCATCCCGCCTC |
| Internal bar code: | CATCCTCGAGTCGCCGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 93.2432 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACGCCTTCACTTCTT |
| Suggested primer 2: | TGTGTGTTGGGTGAAGGAAA |