| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239394 |
| Chromosome: | chromosome 6 |
| Location: | 3349218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277550 | (1 of 3) 3.5.5.1 - Nitrilase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCAGGTCGAAGCAGATGGCGCCACTCA |
| Internal bar code: | TTCAACGGAAGGGTTTTTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 98.4359 |
| LEAP-Seq n confirming: | 944 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTGTAAGAAGTGGGGCG |
| Suggested primer 2: | CCAAATTGAGCCACACACAC |