| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239396 |
| Chromosome: | chromosome 6 |
| Location: | 5754763 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287200 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCATTCGCATCGCACTCGTGGGCTTT |
| Internal bar code: | TTGAGTCAGACACGATGACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 95.0581 |
| LEAP-Seq n confirming: | 654 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTTTGGTGGGTGTGGT |
| Suggested primer 2: | ATAGGGCAGCAATGAAGTGG |