| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239397 |
| Chromosome: | chromosome 13 |
| Location: | 2168429 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578100 | NUP205 | (1 of 1) K14310 - nuclear pore complex protein Nup205 (NUP205, NUP192); Nucleoporin 205 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCCACCTGTGCCCACCGCTCCAGCCGA |
| Internal bar code: | ATTGGCCGTGTGTCTGCACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 70 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGGAGCGAGTGAACACA |
| Suggested primer 2: | ATGGGTGGGTAAAATGCTTG |