| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239401 |
| Chromosome: | chromosome_14 |
| Location: | 2304456 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g623439 | antisense | 3'UTR|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGTAAAGACACAGTGGCCATGCTACTGCG |
| Internal bar code: | CGGGTGCAGTGCCCATCGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 265 |
| LEAP-Seq percent confirming: | 97.7579 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | ATTCACGCACGTGGAATGTA |