| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239438 |
| Chromosome: | chromosome 6 |
| Location: | 7538727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g300650 | (1 of 1) PTHR21373//PTHR21373:SF0 - GLUCOSE REPRESSIBLE PROTEIN MAK10 // N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATCCCAACATACCCCCTCGTCTTCCTG |
| Internal bar code: | CATATAGTAATGCTGTGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 98.3452 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACATGGCTCATTCCCTT |
| Suggested primer 2: | TTGTGGCAGGTCAGACTCAG |