Insertion junction: LMJ.RY0402.239447_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.239447
Chromosome:chromosome 2
Location:1482514
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g083950 PSRP3,cS23 Plastid-specific ribosomal protein 3; (1 of 1) K19032 - 30S ribosomal protein 3 (PSRP3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGATTTATCCTCCACTACCAGCTGTGTTGT
Internal bar code:GCTTCAAGACAGACGCCATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1097
LEAP-Seq percent confirming:99.7002
LEAP-Seq n confirming:8980
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTTCCCTCCCCTCAACTC
Suggested primer 2:GGCTGGGGGTACTAGGAGTC