| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.239466 |
| Chromosome: | chromosome 11 |
| Location: | 2932248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478100 | (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGTACAGGCTCTGCACGGCTGCGACTG |
| Internal bar code: | TCAAGGTTGCTTCGCCGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.8012 |
| LEAP-Seq n confirming: | 502 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACTACTTACACAAGCG |
| Suggested primer 2: | GCAATGGTGTTGGAGACCTT |