| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239493 |
| Chromosome: | chromosome 17 |
| Location: | 4076868 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729350 | (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGCCCTTGCTGCTGCTGCCGCTACCC |
| Internal bar code: | AGAATTGGGGCAGCCGGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 73.8596 |
| LEAP-Seq n confirming: | 421 |
| LEAP-Seq n nonconfirming: | 149 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGTACATGGATGACG |
| Suggested primer 2: | AAGACGTGTGTGACCCCTTC |