Insertion junction: LMJ.RY0402.239502_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.239502
Chromosome:chromosome 7
Location:302995
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g314300 (1 of 1) PF07092 - Protein of unknown function (DUF1356) (DUF1356) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGCTTTCATGGAACGAAATGCGCAAGTC
Internal bar code:GCGAGTTACTGTGGCCTCGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:929
LEAP-Seq percent confirming:98.7872
LEAP-Seq n confirming:2525
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTTTGCCGTTGTGCTTTG
Suggested primer 2:ATGAGGATTGGGAGGAGCTT