| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239541 |
| Chromosome: | chromosome 3 |
| Location: | 7896479 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202350 | (1 of 7) IPR001054//IPR006059//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Solute-binding family 1 // Nucleotide cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTAATATCATAGTGGTTGGCAATGCTCC |
| Internal bar code: | TGAATTTGCAGGCTATATTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 99.5741 |
| LEAP-Seq n confirming: | 4208 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGCCTCCATCATCACTC |
| Suggested primer 2: | ATCAAAATCCCGAAATGCTG |