Insertion junction: LMJ.RY0402.239543_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.239543
Chromosome:chromosome 11
Location:2431203
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g475150 Similar to PKD2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAATGTGCCGGCTGTTTATGGAGTCGAG
Internal bar code:TCGAAACATCTCCCCACCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:785
LEAP-Seq percent confirming:99.3319
LEAP-Seq n confirming:2825
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGAGTTGGAGATGTCGT
Suggested primer 2:GTGTCGGCAAGAGAGGAAAG