Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.239569 |
Chromosome: | chromosome_7 |
Location: | 5574149 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g351650 | FAP20,BUG22 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGTGGGCTGACCGGGTCCGCTTCCTGCA |
Internal bar code: | GGGCACAGGGGAAACGGTAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 282 |
LEAP-Seq percent confirming: | 98.8372 |
LEAP-Seq n confirming: | 85 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTAATAGCCGGCAAGCAAAG |
Suggested primer 2: | TAGCCCTCCCACATTCAGAC |