| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.239601 |
| Chromosome: | chromosome 2 |
| Location: | 3594969 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095137 | PFR1 | (1 of 1) 1.2.7.1 - Pyruvate synthase / Pyruvic-ferredoxin oxidoreductase; Pyruvate ferredoxin oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTTTTCACCTTCCTCGTGCATCCCGCT |
| Internal bar code: | GGCCGAAAATCGACAAACTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATAACACCTGCCCCTCT |
| Suggested primer 2: | TAAACAACACTCACGGCAGC |