| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.239602 |
| Chromosome: | chromosome 4 |
| Location: | 1243322 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215150 | SSS1B,SSS1,SSIb | (1 of 1) IPR023159 - SO1590-like domain; Soluble starch synthase IB | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCATGTGCGCGGCTGTGGTTGATGCGG |
| Internal bar code: | CCTGGATTTCGACCCTGGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 98.8235 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCTGAAGAGGTGTTTGC |
| Suggested primer 2: | CGTAGTCGCACCTCCTTAGC |